Введение
Chapter I. Literature Review
1.1 Theoretical description of DNA 11
1.1.1 Statistical Thermodynamic Models and Molecular Dynamics 11
1.1.2 Polyelectrolyte Models 13
1.2 DNA condensation 22
1.3 Examples of DNA condensing agents 24
1.4 Morphology of condensed particles 28
1.5 Mechanism of condensation 30
1.6 Structure of condensed DNA 32
1.7 Intermolecular forces involved in condensation 34
1.8 Biological significance of condensation 37
1.9 DNA-lipid complexes as a non-viral gene delivery systems 38
Chapter II Materials and methods
II.1. Materials and samples preparation 56
II.2. Physical and biophysical approaches 60
Chapter III Results and discussions
III. 1. Structure of DNA/Dicationic Lipid Complex as revealed by Coherent Phase and Atomic Force Microscopy 72
Ш.2. The Structural-Activity Relationships for Lipoplexes Prepared from a Newly Synthesized Dicationic Lipid and plasmid DNA 85
Ш.З. Tailoring DNA condenstaes using a dicationic lipid with different spacers 94
1ГІ.4. Structural and Morphological Peculiarities of DNA-Vitamin D complexes: A Fluorescence and Atomic Force Microscopy Study 107
Ш.5 A Spectroscopic and Atomic Force Microscopy Study for Oleic 115
Concluding remarks 129
References 132
